- Last Updated on Friday, 04 December 2015 09:35
Ovarian cancer is one of the most lethal gynaecologic malignancies. This can be attributed to the emergence of multi-drug resistance in the clinic due to the over-expression of drug-efflux pumps such as P-gp. To overcome this, we have developed a liposomal platform for the the co-delivery of potent P-gp inhibitors with chemotherapeutic drugs.
“Using time-lapse holographic imaging studies, cell cycle arrest followed by progression to apoptosis
was clearly visualized and confirmed.”
Shravan Kumar Sriraman, Northeastern University
PEGylated liposomes composed of eggphosphatidylcholine, cholesterol and DOTAP loaded with equimolar amounts of tariquidar (XR) and paclitaxel (PCT) were prepared by the thin film hydration method followed by extrusion. The cellular effects of the formulations were evaluated using the holographic time-lapse cytometer HoloMonitor® M4 and iCyte® laser scanning cytometer.