- Last Updated on Saturday, 19 November 2016 10:45
Interfacing antibody-based microarrays and holographic microscopy enables label-free detection of cell volume loss.
“By using holographic microscopy combined with microarray, each cell morphology parameter was available,
not only on population levels, even at the cellular level.”
Zahra El-Schich, Malmö University
Suspension cells are challenging for microscopy since they are floating at several different levels in the media. Even more challenging is to analyze these cells in a non-disturbing way. An antibody microarray can capture and immobilize living, unstained suspension cells. Then morphological parameters such as area, optical thickness, volume and many more can be measured using the HoloMonitor. In this study, after cytotoxic drug treatment of antibody-captured human leukemic T-cells, a time dependent decrease of cell volume, area, thickness, eccentricity and irregularity was observed and quantified.