Holographic microscopy allows changes in cell morphology and cell cycle-related parameters to be monitored non-invasively. The analysis simultaneously includes data for both individual cells and entire populations, including cell cycle duration time and cell cycle phase distribution, allowing non-invasive in vitro cancer treatment studies or toxicology studies.

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“The HoloMonitor allows non-invasive, label-free, monitoring of cancer cells and their re­sponse to treatment, without the need for prior cell extraction, staining or exposing cells to harmful light sources.”
Dr. Maria Falck Miniotis, Malmö University

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Holographic microscopy has been used to reveal significant differences in cell morphology between cells which have been chemically arrested in the G1 or G2 cell cycle phases compared to control cells. Such changes can be detected at much earlier stages using holographic microscopy than other techniques such as flow cytometry.

In this application note we present additional holographic microscopy data and the holographic data from Falck Miniotis study to show that the cell cycle phase distribution correlates well with the flow cytometry method. Additionally, the results of cell cycle duration and correlated morphology studies are presented. All presented holographic microscopy data was recorded and analyzed using HoloMonitor.